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Why is it important to know about ChIP seq?
ChIP-seq is primarily used to determine how transcription factors and other chromatin-associated proteins influence phenotype -affecting mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states.
Which is the most challenging part of chip on chip experiments?
The analysis and information extraction of the raw data often remains the most challenging part for ChIP-on-chip experiments.
When was the chip on chip technology introduced?
Introduced in 2007, ChIP sequencing (ChIP-seq) is a technology that uses chromatin immunoprecipitation to crosslink the proteins of interest to the DNA but then instead of using a micro-array, it uses the more accurate, higher throughput method of sequencing to localize interaction points.
What is the purpose of a chip on a chip?
ChIP-on-chip (also known as ChIP-chip) is a technology that combines chromatin immunoprecipitation ('ChIP') with DNA microarray ( "chip" ). Like regular ChIP, ChIP-on-chip is used to investigate interactions between proteins and DNA in vivo. Specifically, it allows the identification of the cistrome,...
Last Update: Oct 2021
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